Louis, Missouri , USA. Toggle navigation. Login Categories Journals. Publications by authors named “Kevin Guo”. The physical activity and nutrition-related corporate social responsibility initiatives of food and beverage companies in Canada and implications for public health. Epub Jun 9.
Optical Radiology Laboratory (ORL)
Lydia Sauer, Christopher B. Komanski, Alexandra S. Vitale, Eric D. Hansen, Paul S. Fluorescence decays were detected in two spectral channels — nm; — nm to determine fluorescence lifetimes of endogenous fluorophores in their specific spectral emission ranges.
as soon as possible. Download date: different groups of bloodstains were measured using visible reflectance spectroscopy. reflectance, fluorescence or Raman spectroscopy, have the potential to allow on field exponential decay is observed for blood stains with ages between 1 and 20 days, again in.
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You can as find the app-like dating app. He didn’t need-me-want-me-have-to-have-me. Both ill-tempered and passionate, you ll start off disliking and even harshly teasing dating bloodstains with fluorescence lifetime measurements other, but that s nothing a rendition of Can t Take My Eyes Off You and a particularly honest poem can t fix.
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In May , a young woman was found severely injured in the Eltham district of London, her head brutally smashed with a plaster’s hammer. One of the wounds divided the temporal artery, resulting in a large discharge of blood. Several days later, a year old man, her friend and alleged lover, was arrested. Numerous small spots of blood were found on his shirt. The examiner of the case, Dr. Letheby, one of the pioneers of forensic spectral analysis of blood, declined to assign a date to the bloodstains on the shirt and connect the defendant to the murder.
Knowing the time since deposition (TSD) of an evidentiary bloodstain is highly Dating bloodstains with fluorescence lifetime measurements.
In summary, fluorescence lifetime was shown to be a promising technique to evaluate the age of a bloodstain in a crime scene within the first week. The method is fast ca. The major improvement of the technique will come from the development of the lifetime imaging technique, in which the average fluorescence lifetime is evaluated across the region of interest in the bloodstain.
Such an approach will minimize the problems with sampling and provide more accurate results. The measurements can potentially be performed in solid state by using a handheld fluorescence-lifetime instrument, which could be applied to the bloodstain directly at the crime scene with no additional sample handling. Dating bloodstains with fluorescence lifetime measurements. N2 – In summary, fluorescence lifetime was shown to be a promising technique to evaluate the age of a bloodstain in a crime scene within the first week.
AB – In summary, fluorescence lifetime was shown to be a promising technique to evaluate the age of a bloodstain in a crime scene within the first week. Overview Fingerprint. Abstract In summary, fluorescence lifetime was shown to be a promising technique to evaluate the age of a bloodstain in a crime scene within the first week. Keywords Bloodstain age Fluorescence lifetime Forensics Tryptophan.
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WO2017020000A1 – Spectroscopic methods for body fluid age determination – Google Patents
Knowing the time since deposition TSD of an evidentiary bloodstain is highly desired in forensics, yet it can be extremely complicated to accurately determine in practice. Although there have been numerous attempts to solve this problem using a variety of different techniques, currently, no established, well-accepted method exists. Raman spectroscopy, along with two-dimensional correlation spectroscopy 2D CoS and statistical modeling, was used to analyze fresh bloodstains at ten time points under ambient conditions.
The 2D CoS results indicate a high correlation between several Raman bands and the age of a bloodstain. A regression model was built to provide quantitative predictions of the TSD, with cross-validated root mean squared error and R 2 values of 0.
Our approach is based on a fact that the fluorescence lifetime of tryptophan — the major the fluorophore.
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WO2009130580A1 – Apparatus and method for dating a body sample – Google Patents
Herein, we demonstrate a novel method for bloodstain dating based on the fluorescence lifetime of blood. We show that the intrinsic fluorescence lifetime quickly.
Chemists in the US have developed a quick and simple way to find out how old bloodstains are using natural fluorescence measurements. Knowing when blood has been spilled can help forensic scientists pinpoint when a crime took place, but current techniques – such as optical measurements of haemoglobin degradation – can be unreliable. Now, Mikhail Berezin and colleagues at Washington University in St Louis, US, have developed a technique that relies on the fluorescence lifetime of the tryptophan in blood proteins.
Dating bloodstains with fluorescence lifetime measurements.
Augmented longitudinal acoustic trap for scalable microparticle enrichment, Biomicrofluidics , , 12, Zhegalova NG, Gonzales G. Two-photon optical properties of near-infrared dyes at 1. Achilefu S. Multimodality imaging of gene transfer using a receptor-based reporter gene, J.
Bloodstain age analysis: toward solid state fluorescent lifetime measurements. Author(s): Kevin Guo; Date Published: 22 March PDF: 7 pages. Proc.
The present invention relates to an apparatus and method for dating a body sample, preferably, but not exclusively, a sample of body fluid, such as blood, which may be external to the body, for example, having caused stains on an external substrate, such as a floor, wall, etc. It is well known that dating of both external blood stains and internal bruising is a useful forensic technique to determine when a particular injury was caused to a person.
In the one case, the injury would have been such that the skin was punctured and blood was spilt and in the other case no blood was spilt externally, but bruising, to a greater or lesser extent was caused. In both cases, however, there is a need to accurately determine the age of the injury. In general, determination of the age of external blood stains has been carried out by a variety of methods.
Although, historically, this was carried out by very roughly estimating the age based on the colour of the blood stain, more “scientific” methods have recently been employed. Most such methods rely on the fact that, when blood leaves the human body, haemoglobin in the blood is oxidised to met-haemoglobin. Various methods have therefore tried to use different characteristics of haemoglobin and met-haemoglobin to try to determine their relative concentrations and, from those, to try to determine the age of the blood stain.
However, some of these methods require very complicated and advanced technical equipment, so that they cannot easily be done at the scene of the injury, for example a crime scene. In other cases, known techniques often compromise the bloodtraces by using chemicals or by requiring that the stains be taken to a laboratory in order to properly analyse them.
Still others of the methods have proved not to be reliable since they provide too high a deviation compared to the actual age of the stains.